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Bs 29784 prevents the breakdown of the NF-κB inhibitor, IκBα. Caco-2 cells were pretreated apically with Bs 29784 for 16 h prior to basolateral stimulation with IL-1β (20 ng/ml) for 0, 15, 30, or 60 min. Cytolosic levels of IκBα were visualized by Western-blot (A). Band densities were measured using Image J software and subjected to statistical analysis (B). * and **Significant differences between the groups, p < 0.05 and p < 0.01, respectively.
