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. 2019 Mar 29;10:564. doi: 10.3389/fimmu.2019.00564

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Copyright © 2019 Rhayat, Maresca, Nicoletti, Perrier, Brinch, Christian, Devillard and Eckhardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Contribution of soluble and membrane-bound factors to anti-inflammatory effects of Bs 29784. Caco-2 cells were pretreated apically either with live secreting cells (Bs 29784), with PFA-killed Bs 29784 (Cell Associated Factors, CAF) or with Bs 29784 physically separated from the Caco-2 cells by an additional filter (Secreted Factors, SF). After 16 h, the cells were treated basolaterally with IL-1β (20 ng/ml) and IL-8 released in the next 6 h was measured by ELISA. ^* and ^**Significant differences between the groups, p < 0.05 and p < 0.01, respectively.