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. 2019 Mar 29;10:564. doi: 10.3389/fimmu.2019.00564

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Copyright © 2019 Rhayat, Maresca, Nicoletti, Perrier, Brinch, Christian, Devillard and Eckhardt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Blunted cytokine-induced upregulation of iNOS due to pretreatment of cells with Bs 29784. Caco-2 cells were pretreated with Bs 29784: live secreting cells (Bs 29784), PFA-killed cells (CAF), or secreted factors from BS 29784 (SF), or not, and subsequently stimulated basolaterally with Cytomix for 24 h. Cytolosic levels of iNOS were visualized by Western-blot (A). Band densities were measured using Image J software and subjected to statistical analysis (B). ^* and ^**Significant differences between the groups, p < 0.05 and p < 0.01, respectively.