Figure 5. a. Section of the liver of a negative control fish showing closely packed hepatic plates with the hepatocytes not showing visible cytoplasmic vacuoles and with intact bile ducts.

b. The hepatocytes of effluent treated fish contain multiple moderate-sized vacuoles (V), large prominent bile duct with a moderately congested (C) of the central veins and disarrayed hepatocytes.

c. In the effluent treated fish, there are widespread multiple foci of hepatocytes with clear cytoplasmic vacuoles. Also there are widespread large multiple cytoplasmic vacuoles within the hepatocytes, and the central veins moderately congested with disarrayed hepatocytes. Mag. x400.