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. 2019 Mar 29;10:644. doi: 10.3389/fmicb.2019.00644

FIGURE 2.

FIGURE 2

Genomic arrangement and context of target genes (ORFs BOGUAY_2967, 2386 and 0691), with arrows denoting the position of PCR primers pairs including and excluding flanking regions Genomic maps are derived from the GOLD Project ID# Gp0006298 on the IMG database. (A) A subset of the 41659 bp BOGUAY_Contig_00024 contains BOGUAY_0691 gene which translates into a 511aa protein. Primer 5′ ends are shown for pair 3, 0691 ExpF (a, 225 bp upstream of translational start site) and 0691 ExpR (b, ends 38 bp downstream of translational stop codon); and pair 6, 0691F (c, 5′-end at the start codon) and 0691R (d; 5′-end at the end of the gene, excluding the stop codon). (B) A subset of the 12025 bp BOGUAY_Contig_00500 contains the BOGUAY_2967 gene, which translates into a 671aa protein. Primer 5′ ends are shown for primer pair 1, 2967 ExpF (a; 184 bp upstream of translational start site) and 2967 ExpR (b; ends 281 bp downstream of translational stop codon); and for primer pair 4, 2967F (c, 5′-end at the start codon) and 2967 R (d; 5′-end at the end of the gene, excluding the stop codon). (C) A subset of the 15900 bp BOGUAY_Contig_01341 contains the BOGUAY_2386 gene, which translates into a 582aa protein. Primer 5′ ends are shown for pair 2, 2386 ExpF (a, 40 bp upstream of translational start site), and 2386 ExpR (b, ends 91 bp downstream of translational stop codon); and for primer pair 5, 2386F (c, 5′-end at the start codon) and 2386R (d, position 5′-end at the end of the gene, excluding the stop codon).