Table 3.
Spectrophotometric nitrite reductase enzymatic assays of cell lysates and partially purified proteins.
| ORF | Gene expression sample | Activity |
|---|---|---|
| BOGUAY_2386 | ONR E2-6-14-2016 | 1.287 ± 0.0016 |
| Purified protein, second elution | ||
| BOGUAY_2967 | nirS cell lysate + IPTG | 2.624 ± 0.64 |
| BOGUAY_2967 | nirS cell lysate – IPTG | 0.0009 ± 0.0008 |
| BOGUAY_0691 | Orange Protein E1 | 0.0144 ± 0.0025 |
| Purified protein | ||
| BOGUAY_0691 | Orange Protein E2 | 0.0028 ± 0.0014 |
| Purified protein | ||
| BOGUAY_0691 | Original orange protein sample (MacGregor et al., 2013b) | 0.052 ± 0.025 |
| BOGUAY_0691 | Partially purified sample (MacGregor et al., 2013b) | 0.31 ± 0.1 |
Activity is given in μmol min-1 mg protein-1. The negative controls for BSA and the cell lysate of the expression strain pET22b showed activities of 0.0005 and 0.0003 μmol min-1 mg protein-1, respectively. For all assays, activities are shown as units ± standard deviation (n ≥ 3).