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. 2019 Mar 20;10:228. doi: 10.3389/fphys.2019.00228

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Copyright © 2019 Yang, Shao, Zhu, Li, Zhang, Ding, Wu, Xu, Yue, Tang, Kuang, Fan, Zhu and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mTORC1 signaling was activated by irradiation in liver tissues. (A and B) Expression of pS6 was upregulated post-irradiation in liver tissues. Expression of pS6 and S6 (A) was detected by western blotting at days 1, 3, and 7 after irradiation (n = 3). GAPDH was used as a housekeeping control. Expression of pS6 and S6 (B) was quantitated by ImageJ software. (C and D) pS6 immunostaining in liver tissues (n = 6). Liver tissues were fixed in 4% paraformaldehyde after irradiation and pS6 immunostaining was performed as indicated in materials and methods (C). Scale bar = 50 μm. Arrows indicate pS6 positive cells. Imaging software was used to analyze the integrated optical density (IOD) of pS6 (D). ***p < 0.001 vs. CTL.