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. 2019 Mar 20;10:228. doi: 10.3389/fphys.2019.00228

Figure 4.

Figure 4

Rapamycin treatment reduced the increase of pS6 expression in irradiated liver tissues. (A and B) Increased expression of pS6 and p-mTOR by irradiation was reduced through rapamycin (Rap) treatment. Expression of pS6, S6, p-mTOR, and mTOR (A) was detected by western blotting at day 7 after irradiation (n = 3). GAPDH was used as a housekeeping control. Expression of pS6, S6, p-mTOR, and mTOR (B) was quantitated by ImageJ software. The ratios of pS6/S6 and p-mTOR/mTOR are presented. (C and D) pS6 immunostaining in liver tissues (n = 6). Liver tissues with or without rapamycin treatment were harvested at day 7 post-irradiation and fixed in 4% paraformaldehyde after irradiation, and pS6 immunostaining was performed as indicated in materials and methods (C). Scale bar = 50 μm. Arrows indicate pS6-positive cells. Imaging software was used to analyze the integrated optical density (IOD) of pS6 (D). **p < 0.01, ***p < 0.001 vs. Veh.