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. 2019 Mar 22;10:100203. doi: 10.1016/j.bonr.2019.100203

Fig. 1.

Fig. 1

Number of CXCL12 abundant reticular cells and SDF-1 production in YF bone marrow. Flow analysis of CAR cells and SDF-1 production. A. Total bone marrow cells were gated for a population negative for CD45, Ter119, CD31, and Sca1 and positive for VCAM and Pdgfr, the phenotype of CAR cells. B. Number of CAR cells in bone marrow of 8 week-old WT and YF male mice. Percentage of CAR cells in bone marrow was calculated as the number in YF bone marrow relative to WT bone marrow. Data is pooled from five independent experiments (WT, n = 25; YF n = 27). C. Intracellular level of SDF-1 was determined by the mean fluorescence intensity (factor of mean SDF-1 staining and SDF-1 positive cell count). Histogram in grey shade represents mouse IgG FITC isotype control. Bold black line represents SDF-1 FITC stain in YF cells and thin grey line represents in WT cells. D. SDF-1 intracellular level is shown as amount of protein in CAR cells in YF bone marrow relative to CAR cells in WT bone marrow (n = 9 mice/genotype). E. qRT-PCR analysis of SDF-1 mRNA levels in FACS sorted CAR cells from bone marrow (n = 9 mice/genotype). *P value of <0.05 vs. WT.