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. 2019 Mar 22;10:100203. doi: 10.1016/j.bonr.2019.100203

Fig. 2.

Fig. 2

Increased PI3K activity and increased transcription of SDF-1 in the absence of Cbl-PI3K interaction. A. qRT-PCR analysis of SDF-1 mRNA levels in magnetically sorted CD45 Ter119 CD31 Sca1 negative population of bone marrow cells, cultured and treated with PI3K inhibitor LY294002 at indicated concentrations for 3 h. SDF-1 mRNA levels were normalized to GAPDH, and values shown are relative to the SDF-1 mRNA levels in untreated WT cells. B. Western blotting analysis of AKT phosphorylation, indicative of PI3K activity. Bands were quantified by LICOR odyssey software and the ratio of pAKT to total AKT at indicated concentrations of PI3K inhibitor, LY294002 is shown below the blots with values relative to untreated WT cells. C. qRT-PCR analysis of SDF-1 mRNA levels following treatment with an AKT inhibitor at the indicated concentrations D. Western blotting analysis of GSK phosphorylation, indicative of AKT activity. A representative of 3 independent experiments is shown and P value of <0.05 vs. WT; *compared to untreated WT cells, # compared to untreated YF cells.