Table 1.
Bacterial strains and plasmids used in present study.
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| XL-Blue | E. coli (recA1 endA1 gyrA96 (NalR) thi-1 hsdR17 (rk_ mk_)glnV44 relA1 lac [F’::Tn10(TetR)'proAB lacIq Δ(lacZ)M15]) | Stratagene |
| CC118 (λpir+) | E. coli Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recAl, lysogenized with λpir phage | Herrero et al. (1990) |
| MG1655 | E. coli K12 wild-type | VKPM B-6195 |
| MG1655 (Δφ80-attBnative)φ80-attB trs5-7 | E. coli K-12 MG1655 with deleted native φ80-attB site and φ80-attB in trs5-7 locus | Minaeva et al. (2008) |
| MG1655 4Δ | MG1655 ΔilvE ΔtyrB ΔavtA::KmR ΔaspC::TetR | Laboratory collection |
| BW25113 ΔilvE::KmR | E. coli K12 Δ(araD-araB)567 Δ(rhaD-rhaB)568 ΔlacZ4787 (::rrnB-3) hsdR514 rph-1 lacI+ ΔilvE::FRT-kan-FRT | Keio Collection |
| Baba et al. (2006),Grenier et al. (2014) | ||
| MG1655 trs5-7::bcd-TetR | MG1655 (Δφ80-attBnative) trs5-7::bcd-λattL-TetR- λattR | Present study |
| MG1655 trs5-7::bcd | MG1655 (Δφ80-attBnative) trs5-7::bcd -TetS | Present study |
| MG1655 trs5-7::bcd ΔilvE::KmR | MG1655 (Δφ80-attBnative) trs5-7::bcd -TetS ΔilvE:: FRT-kan-FRT | Present study |
| MG1655 cat-PL-bcd5.7 | MG1655 (Δφ80-attBnative) trs5-7:: λattL-CmR- λattR-PL-bcd | Present study |
| MG1655 cat-PL-ilvE5.7 | MG1655 (Δφ80-attBnative) trs5-7:: λattL-CmR- λattR- PL-ilvE ΔilvE:: FRT-kan-FRT | Present study |
| B-7 ΔilvBN ΔilvGM ΔilvIH | E. coli K12 ΔilvBN ΔilvGM ΔilvIH | Sycheva et al. (2009) |
| B-7 ΔilvIH ΔilvGM cat-PL-ilvBNN17K | E. coli K12 ΔilvIH ΔilvGM λattL-CmR- λattR-PL- ilvBNN17K | Sycheva et al. (2009) |
| MG1655 cat-PL-ilvBNN17KDA | MG1655ΔilvGME::λattL-CmR- λattR-PL- ilvBNN17KDA | Laboratory collection, Serebrianyi |
| B-7 ΔilvBN ΔilvGM ΔilvIH cat-PL-ilvBNN17KDA | E. coli K12 ΔilvIH ΔilvBN ΔilvGME:: λattL-CmR- λattR-PL-ilvBNN17KDA | Present study |
| V1 | E. coli K12 ΔilvIH ΔilvBN ΔilvGME::PL-ilvBNN17KDA-CmS | Present study |
| V1 cat-PL-bcd5.7 | E. coli K12 ΔilvIH ΔilvBN | Present study |
| ΔilvGME::PL-ilvBNN17KDA trs5-7:: λattL-CmR- λattR-PL-bcd | ||
| V1 cat-PL-ilvE5.7 | E. coli K12 ΔilvIH ΔilvBN ΔilvGME::PL-ilvBNN17KDA trs5-7:: λattL-CmR- λattR- PL-ilvE | Present study |
| Plasmids | ||
| pUC57-bcd-Bsub | pMB1 ori; AmpR; bcd | Present study |
| pMW118 | pSC101 ori; AmpR; MCS | GenBank accession number AB005475 |
| pMW118-bcd | pSC101 ori; AmpR; Plac-bcd | Present study |
| pAH162- λattL-TetR- λattR -2Ter | oriR; λattL-TetR- λattR; attP φ80; MCS | Minaeva et al. (2008) |
| pAH162- λattL-TetR- λattR-2Ter-bcd | oriR; λattL-TetR- λattR; attP φ80; bcd | Present study |
| pAH123 | oriR101, repA101ts, λcIts857, λPR→φ80-int, AmpR | Haldimann and Wanner (2001), GenBank accession number AY048726 |
| pKD46 | oriR101, repA101ts, araC, ParaB-[γ, β, exo of phage λ], AmpR | Datsenko and Wanner (2000), GenBank accession number AY048746 |
| pMW-Int-Xis | oriR101, repA101ts, λcIts857, λPR→λxis-int, AmpR | Minaeva et al. (2008) |