Table 2.
Oligonucleotides used in this study.
| Primers | Sequence (5′- 3′) | Purpose |
|---|---|---|
| P1 | AAAGGATGAACTACGAGGAAGGGAACAACATTC-ATACGCTCAAGTTAGTATAAAAAAGCTGAAC | creation of cat-PL-ilvBNN17K |
| P2 | ATGGGACGGTGCGTGCCGTCCCATTTTTTGTATTTACTGAAAAAACACCGCGATCTTGTTAAAC | creation of cat-PL-ilvBNN17K |
| P3 | GTAAAGCGCTTACGCGTCGA | verification of cat-PL-ilvBNN17K integration |
| P4 | TGCAAGTGAAGTTGAGTTGTTC | verification of cat-PL-ilvBNN17K integration |
| P5 | GAATGATATCCATATCCTCGAC | verification of bcd integration |
| P6 | GTCTTCTCACGGGAACGGTT | verification of bcd integration |
| P7 | CGAAAGTGATTGCGCCTACCCGGATATTATCGTGAGCGCTCAAGTTAGTATAAAAAAGCTGAAC | creation of cat-PL upstream bcd and ilvE |
| P8 | TATATTTAAAAAGTTCCATACATAGATCTCCTTCTTCGGCCAATGCTTCGTTTCGTATCACACA | creation of cat-PL upstream bcd |
| P9 | CCATATTACGACCATGAGTGG | verification of ilvE deletion |
| P10 | CCAGTAATTCAGAAATGTTGG | verification of ilvE deletion |
| P11 | AGATAGATCTCCTTCTTCGGCCAATGCTTC | creation of cat-PL upstream of ilvE |
| P12 | ATTGGCCGAAGAAGGAGATCTATCTATGACCACGAAGAAAGCTGATTACATTT | amplification of ilvE |
| P13 | AAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCTTATTGATTAACTTGATCTAACCAGCCC | amplification of ilvE |
| P14 | GTTCGTTGCAACAAATTGATAAG | sequencing of PL-ilvE |
| P15 | CAGGGAAGAGAGGTAGTTACC | sequencing of PL-ilvE |
| P16 | GATCGATGCGATGGTTTCCTC | sequencing of PL-ilvE |