Table 6.
Valine accumulation by Bcd- or IlvE- harboring E. coli strains under different aeration conditions.a
| Strain | O2 conditionsb | Val, g/L | OD540 | Glc residual, g/L | Y m/m, % |
|---|---|---|---|---|---|
| V1 cat-PL-bcd5.7 | microaerobic | 9.1 ± 0.3 | 19.5 ± 0.3 | 20.3 ± 0.4 | 35.3 ± 0.8 |
| aerobic | 6.9 ± 0.1 | 34.5 ± 0.8 | 0 | 17.7 ± 0.3 | |
| V1 cat-PL-ilvE5.7 | microaerobic | 4.1 ± 0.1 | 14.5 ± 0.3 | 24.6 ± 0.3 | 17.8 ± 0.3 |
| aerobic | 9.8 ± 0.4 | 31.3 ± 1.8 | 0 | 25.1 ± 1.1 |
a
Cultivation time was 48 h; data represent the means of three separate experiments; kLa was determined by sulfite method as described in p2.10 “Materials and methods”.
b
Microaerobic conditions (kLa = 5.4 ± 0.2 × 10−4, mmole O2 ml−1 min−1); aerobic conditions (kLa = 14.2 ± 0.4 × 10−4, mmole O2 ml−1 min−1).