Fig. 5. CHOP is involved in DHA-mediated apoptosis in SNU-601/cis2 cells.

SNU-601/cis2 cells transfected with shRNAs specific for CHOP or EGFP were treated with 200 μM DHA for 12 h (A) or 24 h (B, C). The cell lysates were subjected to SDS-PAGE, followed by immunoblot analyses using antibodies specific for CHOP and GAPDH (A) and PARP, caspase-7, and GAPDH (B). The cell viability was measured using the MTT assay. Significant differences have been indicated as *p < 0.05 (C).