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. 2019 Mar 29;10:584. doi: 10.3389/fmicb.2019.00584

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Copyright © 2019 Flodman, Tsai, Xu, Corrêa, Copelas, Lee, Xu, Weigele and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ligation of phages ViI and phi W-14 restriction fragments by T4 DNA ligase. (A) Ligation of phage ViI restriction fragments partially digested by NlaIII and FatI, and completely digested by RsaI. Cleavage sites of REases: NlaIII (CATG/), 3′ 4-base overhang; RsaI (GT/AC), blunt ends; FatI (/CATG), 5′ 4-base overhang. (B) Ligation of phage phi W-14 restriction fragments generated by RsaI, Sau3AI, and MboI digestions. Cleavage sites of RsaI (GT/AC), blunt ends; Sau3AI (/GATC), and MboI (/GATC), 5′ 4-base overhang.