Table 1.
Common genes in the transcriptomes of carpet glia, adult surface glia, and the mouse BBB.
| Category | Term | Gene name (symbol) | Average log2 FC | Molecular function | Mouse BBB gene Gene name (symbol) |
|---|---|---|---|---|---|
| CELL ADHESION MOLECULES | |||||
| GOTERM_MF_DIRECT | GO:0050839~cell adhesion molecule binding | midline fasciclin (mfas) | 1.26 | GO:0050839~cell adhesion molecule binding | |
| GOTERM_BP_DIRECT | GO:0007155~cell adhesion | Rho-Like (RhoL) | 1.07 | GO:0003924~GTPase activity | |
| held out wings (how) | 0.72 | GO:0003730~mRNA 3′-UTR binding | |||
| CAP | 0.72 | InterPro: IPR028516~actin filament organization | Sorbin and SH3 domain containing 2 (Sorbs2) | ||
| scab (scb) | 0.70 | GO:0050839~cell adhesion molecule binding | |||
| pebble (pbl) | 0.60 | GO:0005096~GTPase activator activity | |||
| Notch (N) | 0.54 | GO:0004888~transmembrane signaling receptor activity | |||
| GOTERM_BP_DIRECT | GO:0019991~septate junction assembly | lethal (2) giant larvae (l(2)gl) | 0.83 | GO:0005096~GTPase activator activity | LLGL1 scribble cell polarity complex component (Llgl1) |
| locomotion defects (loco) | 0.79 | GO:0001965~G-protein alpha-subunit binding | Regulator of G-protein signaling 5 (Rgs5) | ||
| moody | 0.70 | GO:0004930~G-protein coupled receptor activity | |||
| Neuroglian (Nrg) | 0.60 | GO:0005509~calcium ion binding | F11 receptor (F11r) | ||
| coracle (cora) | 0.55 | GO:0005200~structural constituent of cytoskeleton | |||
| GOTERM_CC_DIRECT | GO:0005912~adherens junction | Moesin (Moe) | 1.43 | GO:0003779~actin binding | |
| Shroom | 0.84 | GO:0003779~actin binding | Shroom family member 4 (Shroom4) | ||
| shotgun (shg) | 0.76 | GO:0008013~beta-catenin binding | |||
| warts (wts) | 0.75 | GO:0004674~protein serine/threonine kinase activity | |||
| karst (kst) | 0.59 | GO:0008092~cytoskeletal protein binding | |||
| GOTERM_CC_DIRECT | GO:0005925~focal adhesion | blistery (by) | 1.25 | GO:0003779~actin binding | |
| inflated (if) | 0.74 | GO:0050840~extracellular matrix binding | |||
| Vinculin (Vinc) | 0.65 | GO:0003779~actin binding | Catenin, cadherin associated protein, alpha 1 (Ctnna1) | ||
| alpha actinin (Actn) | 0.60 | GO:0003779~actin binding | |||
| Paxillin (Pax) | 0.60 | GO:0008270~zinc ion binding | |||
| TRANSPORTERS | |||||
| GOTERM_BP_DIRECT | GO:0006810~transport | Acyl-CoA binding protein 2 (Acbp2) | 1.39 | GO:0000062~fatty-acyl-CoA binding | Diazepam binding inhibitor (Dbi) |
| CG6836 | 1.17 | GO:0005215~transporter activity | |||
| CG3091 | 1.04 | GO:0005215~transporter activity | |||
| bloated tubules (blot) | 0.95 | GO:0005215~transporter activity | |||
| CG3792 | 0.90 | UniProtKB:O75352~protein glycosylation | |||
| CG12004 | 0.69 | GO:0005215~transporter activity | |||
| inebriated (ine) | 0.60 | GO:0005215~transporter activity | |||
| bedraggled (bdg) | 0.56 | GO:0005215~transporter activity | |||
| INTERPRO | IPR013525:ABC-2 type transporter | CG3164 | 1.13 | GO:0016887~ATPase activity | ATP binding cassette subfamily G member 2 (Abcg2) |
| CG9990 | 1.01 | GO:0016887~ATPase activity | ATP binding cassette subfamily G member 2 (Abcg2) | ||
| CG31689 | 0.76 | GO:0016887~ATPase activity | ATP binding cassette subfamily G member 2 (Abcg2) | ||
| INTERPRO | IPR020846:Major facilitator superfamily domain | Picot | 1.39 | GO:0015293~symporter activity | |
| Organic anion transporting polypeptide 74D (Oatp74D) | 0.91 | GO:0005215~transporter activity | Solute carrier organic anion transporter family, member 2b1 (Slco2b1) | ||
| CG8034 | 0.89 | GO:0008028~monocarboxylic acid transmembrane transporter activity | Solute carrier family 16, monocarboxylic acid transporter, member 4 (Slc16a4) | ||
| CG15890 | 0.78 | InterPro: IPR011701~transmembrane transport | Solute carrier family 16, member 3 (Slc46a3) | ||
| CG6231 | 0.72 | GO:0015101~organic cation transmembrane transporter activity | Solute carrier family 22, organic anion transporter, member 8 (Slc22a8) | ||
| CG3168 | 0.71 | GO:0008514~organic anion transmembrane transporter activity | Solute carrier family 2, facilitated glucose transporter, member 1 (Slc2a1) | ||
| Organic cation transporter 2 (Orct2) | 0.71 | GO:0022857~transmembrane transporter activity | Solute carrier family 22, organic anion transporter, member 8 (Slc22a8) | ||
| CG4797 | 0.58 | GO:0022857~transmembrane transporter activity | |||
| CG3036 | 0.58 | GO:0022857~transmembrane transporter activity | |||
| CG6126 | 0.52 | GO:0005275~amine transmembrane transporter activity | Solute carrier family 22, organic anion transporter, member 8 (Slc22a8) | ||
| CG8051 | 0.50 | GO:0008028~monocarboxylic acid transmembrane transporter activity | Solute carrier family 16, monocarboxylic acid transporter, member 1 (Slc16a1) | ||
| DRUG METABOLISM | |||||
| KEGG_PATHWAY | dme00040:Pentose and glucuronate interconversions | Glutathione S transferase S1 (GstS1) | 1.66 | GO:0004364~glutathione transferase activity | Glutathione S-transferase, mu 2 (Gstm2) |
| GOTERM_BP_DIRECT | GO:0006098~pentose-phosphate shunt | Chloride intracellular channel (Clic) | 1.55 | GO:0005254~chloride channel activity | |
| KEGG_PATHWAY | dme00480:Glutathione metabolism | Glutathione S transferase D9 (GstD9) | 1.52 | GO:0004364~glutathione transferase activity | |
| GOTERM_MF_DIRECT | GO:0004602~glutathione peroxidase activity | Glutathione S transferase D1 (GstD1) | 1.44 | GO:0004364~glutathione transferase activity | |
| GOTERM_MF_DIRECT | GO:0004364~glutathione transferase activity | CG9436 | 1.42 | GO:0004032~alditol:NADP+1-oxidoreductase activity | Aldo-keto reductase family, member C14 (Akr1c14) |
| GOTERM_BP_DIRECT | GO:0006749~glutathione metabolic process | CG3609 | 1.39 | GO:0016491~oxidoreductase activity | |
| INTERPRO | IPR010987:Glutathione S-transferase, C-terminal-like | CG10863 | 1.29 | GO:0004032~alditol:NADP+1-oxidoreductase activity | Aldo-keto reductase family, member C14 (Akr1c14) |
| INTERPRO | IPR004046:Glutathione S-transferase, C-terminal | Glutathione S transferase E3 (GstE3) | 1.28 | GO:0004364~glutathione transferase activity | |
| INTERPRO | IPR004045:Glutathione S-transferase, N-terminal | Phosphogluconate dehydrogenase (Pgd) | 1.19 | GO:0004616~phosphogluconate dehydrogenase activity | |
| KEGG_PATHWAY | dme00982:Drug metabolism-cytochrome P450 | Thioredoxin peroxidase 1 (Jafrac1) | 1.15 | GO:0004602~glutathione peroxidase activity | |
| KEGG_PATHWAY | dme00980:Metabolism of xenobiotics by cytochrome P450 | Glutathione S transferase E6 (GstE6) | 1.17 | GO:0004364~glutathione transferase activity | |
| Microsomal glutathione S-transferase-like (Mgstl) | 1.17 | GO:0004364~glutathione transferase activity | |||
| CG30022 | 0.97 | GO:0005506~iron ion binding | |||
| CG30499 | 0.88 | GO:0004750~ribulose-phosphate 3-epimerase activity | |||
| Glutamate-cysteine ligase catalytic subunit (Gclc) | 0.71 | GO:0004357~glutamate-cysteine ligase activity | |||
| CG17323 | 0.59 | GO:0015020~glucuronosyltransferase activity | |||
| CG6084 | 0.59 | GO:0004032~alditol:NADP+1-oxidoreductase activity | Aldo-keto reductase family, member C14 (Akr1c14) | ||
| UDP-glycosyltransferase family 35 member A1 (Ugt35A1) | 0.54 | GO:0008194~UDP-glycosyltransferase activity | |||
All genes annotated in distinct GO term categories, KEGG pathways, and Interpro are listed. Genes in the fields of cell adhesion molecules and transporters are further divided based on their annotated GO terms. Genes in the field of drug metabolism are listed without further division to avoid redundancy. For simplicity, only one GO_MF term for each gene is displayed. For details, please see Table S3. BBB, blood–brain barrier.