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. 2019 Mar 29;10:635. doi: 10.3389/fimmu.2019.00635

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Copyright © 2019 Moonen, Hirschfeld, Cheng, Chapple, Loos and Nicu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adhesion by cPMNs and oPMNs. Micrographs of FITC labeled bacterial adhesion by cPMNs (A) and oPMNs (B). Arrows depict adhesion of FITC labeled bacteria (Fusobacterium nucleatum) which are shown in green. All micrographs are representatives of 3 independent experiments. Scales represent 50 μm. Quantitative analysis of adhesion of heat-inactivated A. actinomycetemcomitans [Aa, (C)], P. gingivalis [(Pg, (D)], F. nucleatum [Fn, (E)], T. forsythia [Tf, (F)], S. mutans [Sm, (G)], S. Sanguinis [Ss, (H)], E. coli [Ec, (I)], and C. albicans [Ca, (J)] by cPMNs (white bars) and oPMNs (gray bars). The gating strategy presented in Supplementary Figure 3 was employed for all quantifications. Data are presented as mean (+SEM) percentages of adhesion, note the variation in y-axis scales. n = 3, ^** p < 0.01.