Effects of HHT, IPI504, HHT+ IPI504 on cell cycle and colony formation. (A and B) The representative flow cytometry analysis for PI staining in MV4-11 and MOLM-13 cell lines treated with HHT, IPI504 or HHT+IPI504 at the indicated concentrations for 48 h are depicted. (C and D) Representative images of colonies formation for MV4-11 and MOLM-13 cells under HHT (2.5 nM), IPI504 (5 nM) and HHT+IPI504 treatment about 14 days. Quantification of colony counts (% of control) with different group treatment as indicated. (mean ± SEM, n=3, *P < .05, **P < .01, ***P < .001)