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. 2019 Feb 28;12(4):772–786. doi: 10.1016/j.stemcr.2019.01.022

Figure 3.

Figure 3

Nicotine Reduces Cell Viability, Increases ROS Levels, and Changes Cell Cycle in EBs

(A) Representative bright-field images of day 21 control and nicotine-exposed EBs. Scale bar, 100 μm.

(B) Size measurement of control (n = 45) and nicotine-exposed (n = 43) EBs in terms of diameter. EBs were collected from three independent EB differentiation experiments and pooled together for size measurement. ∗∗∗p < 0.001.

(C) Cell viability assay of EBs based on quantitation of the ATP present in control and nicotine-exposed EBs. Cell viability were measured from three independent experiments. ∗p < 0.05.

(D) Reactive oxygen species (ROS) generation in control and nicotine-exposed EBs. ROS was measured from three independent experiments. ∗p < 0.05.

(E) Proportion of cluster 13 muscle cells in G2M, S, or G1 phase.

(F) Distribution of TUBB4B expression in three cell-cycle phases in control (top) and the nicotine-exposed EBs (bottom). Dashed lines represent the center of G2M phase in control and nicotine-exposed EBs.