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qPCR validation of the RNA-seq data using 9 random selected lncRNA. The elongation factor 1-γ (EF1-γ) gene was used as the reference gene. The relative expression level of lncRNAs was calculated relative to its corresponding mock sample. Error bars represented the standard error of 2 biological replicates. lncRNA indicates long non-coding RNA; qPCR, quantitative polymerase chain reaction; RNA-seq, RNA-sequencing.
