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. 2019 Mar 7;12(4):787–800. doi: 10.1016/j.stemcr.2019.02.002

Figure 5.

Figure 5

Expression of Mature β Cell Markers in HiPEC-1-Derived Grafts

(A) Cell composition quantification by high content image analysis for insulin, NKX6-1, and MAFA. Values were normalized to DAPI-positive cells (n = 6 animals).

(B) qRT-PCR analyses for MAFA transcript in hiPSCs (n = 2 lines), PE-D12 (n = 6 differentiations), explants (n = 4), and human islets (n = 2 donors). Error bars indicate SEM.

(C) Extracted immunofluorescence staining from high content imaging for MAFA (red), NKX6-1 (green), and INSULIN (white). The three yellow arrowheads point to three cells showing triple-positive staining. Scale bar, 50 μm.