Figure 6.

Physiological Oxygen Concentrations Reveal a Pro-glycolytic Metabolism in the Absence of p62

(A) Representative western blot analysis of Hif-1α and p62 steady-state levels in Co1^NES and Pat1^NES cells, cultured for 0, 8, or 24 h at 3% O₂. Tubulin was used as a loading control (n = 3).

(B) Quantification of (A). Data are expressed as mean ± SD and differences were tested by a two-tailed t test. ^∗∗p < 0.01, ^∗∗∗p < 0.001, n = 3.

(C) Western blot analysis of HK2, PDK1, PDK3, and LDHA as in (A). Tubulin was used as a loading control. n = 3.

(D) Western blot analysis of Hif-1α expression in Co1^NES and Pat1^NES cells, cultured for 0, 5, or 24 h in the presence of 200 μM CoCl₂. Tubulin was used as loading control. n = 5.

See also Figure S6.