FIGURE 1.

Evaluation of genotyping method using the Gouvea and new primer sets. Japanese representative RVA strains were evaluated by performing RT-PCR (first PCR) and multiplex-PCR (second PCR) with the Gouvea primer set (A) or our new primer set (B). The PCR products were analyzed by electrophoresis on 1.5% agarose gels. Estimated product sizes by each primer set are shown in the table to the right. Arrows indicate mistyped cases (equine-like G3 and G8), a difficult to detect case (G9 lineage 3) and a case showing a non-specific band (G9 lineage 6).