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. 2019 Mar 29;10:250. doi: 10.3389/fphys.2019.00250

Figure 3.

Figure 3

Rap-Osx mice showed repressed odontoblast proliferation, differentiation and DSPP secretion. (A,B) The protein expression of DSPP in developmental teeth of Rap-Osx mice and Ctrl mice. (D,E) The protein expression of DMP1 in developmental teeth of Rap-Osx mice and Ctrl mice. (G,H) The protein expression of OCN in developmental teeth of Rap-Osx mice and Ctrl mice. (J,K) The protein expression of Ki67 in developmental teeth of Rap-Osx mice and Ctrl mice. (C,F,I) The protein expression levels of DSPP, DMP1 and OCN in developmental teeth of Rap-Osx mice and Ctrl mice. (L) Quantitative analysis of the Ki67 positive dental mesenchymal cells in developmental teeth of Rap-Osx mice and Ctrl mice. (M,N) Alizarin red staining was performed on primary cultured dental mesenchymal cells from Ctrl and Rap-Osx mice after osteogenesis induction for 21 days. (O) Quantitative analysis of the relative differentiation ability, i.e., calcium knot formation ability. Data represent the mean ± SD (*p < 0.05, **p < 0.001, ***p < 0.0001).