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. 2019 Mar 29;10:250. doi: 10.3389/fphys.2019.00250

Figure 5.

Figure 5

Phosphorylation capacity of pS6K1 decreased after Raptor deficiency. (A) Detection of phosphorylation levels of pS6K1 in the mandibular first molar was performed by immunohistochemistry staining at P0 (a), P7 (b), P14 (c), and P28 (d). (B) Detection of the phosphorylation levels of pS6 in the mandibular first molar was performed at P0 (e), P7 (f), P14 (g), and P28 (h). (C,D) Detection of phosphorylated S6 in the mandibular first molar at P0. (E,F) Detection of phosphorylated 4E-BP1 in the mandibular first molar at P0. DP, dental pulp; OB, odontoblasts; D, dentin. (G) Protein expression levels of p-S6K1 and p-S6 in WT and mutant mice. (H) The DSPP expression level decreased by the S6K1 inhibitor. (I) PF-4708671 inhibited the proliferation of dental mesenchymal cells from WT mice.