Figure 5.

WDR5, BARD1, and BRCA1 Are Functionally Connected in the DNA Damage Response Pathway

(A) Representative FACS histograms showing the cell distribution with log-intensity of γH2A.X in reprogramming populations measured in different conditions (white, nt; purple, siRNA). Each sample was measured in at least three independent experiments.

(B) Dot plot representing the quantification of γH2AX-positive cells in each condition. Data points correspond to biological replicates from independent experiments. Statistical significance was determined by one-way ANOVA (^∗p < 0.05, ^∗∗p < 0.005, ^∗∗∗p < 0.0005).

(C) Confocal images of reprogramming cells at day 3, stained for γH2A.X (green) OCT4 (red), and DNA (DAPI, blue). Scale bars, 100 μm (left-middle). Zoom-in (right): magnification from inset, showing characteristic γH2A.X foci in all samples except nt control.