Table 5.
Analysis of inter-species MBW pairwise interaction by LUMIER pulldown assays.
| WDR | bHLH | MYB | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AtTTG1 | AtGL3 | AtEGL3 | AtTT8 | AtMYC1 | AtGL1 | AtWER | AtPAP1 | AtPAP2 | AtTT2 | AtMYB61 | w/o | ||
| AaTTG1 | +/+ | +/+ | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| GhTTG1 | +/+ | +/+ | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| GhTTG2 | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| GhTTG3 | +/w | +/+ | -/- | +/+ | -/- | -/- | -/- | -/- | w/w | -/- | -/- | ||
| WDR | GhTTG4 | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | |
| PhAN11 | +/+ | +/+ | -/- | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| ZmPAC1 | +/+ | +/+ | +/+ | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| ZmMP1 | -/- | -/- | -/- | w/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | ||
| AaGL3 | +/+ | +/+ | +/+ | +/+ | w/+ | +/+ | -/- | -/- | |||||
| AaEGL3 | +/+ | +/+ | +/+ | w/w | -/- | +/+ | -/- | -/- | |||||
| AaTT8 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/w | -/- | |||||
| AaMYC1 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | -/- | |||||
| bHLH | GhDEL61 | +/+ | +/+ | +/+ | +/+ | +/w | +/+ | -/- | -/- | ||||
| GhDEL65 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | -/- | |||||
| PhAN1 | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | -/- | |||||
| PhJAF13 | +/+ | +/+ | +/+ | +/+ | w/w | +/+ | -/- | -/- | |||||
| ZmR(Lc) | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | -/- | |||||
| ZmR(S) | +/+ | +/+ | +/+ | +/+ | +/+ | +/+ | -/- | -/- | |||||
| ZmB | +/+ | -/- | -/- | -/- | -/- | -/- | -/- | -/- | |||||
| AaGL1 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| AaWER | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| AaPAPL | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| AaMYB23 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| GhMYB2 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| GhMYB3 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| GhMYB25 | -/- | -/- | -/- | -/- | -/- | -/- | |||||||
| MYB | GhRLC1 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | ||||||
| PhAN2 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| PhAN4 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| PhPH4 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| ZmC1 | -/- | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| ZmPL | w/w | +/+ | +/+ | +/+ | +/+ | -/- | |||||||
| ZmP1 | -/- | -/- | -/- | -/- | -/- | -/- | |||||||
| w/o | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | -/- | - |
Arabidopsis thaliana [At], Arabis alpine [Aa], Gossypium hirsutum [Gh], Petunia hybrid [Ph] and Zea mays [Zm]. All proteins are single-expressed in human cells (HEK293TN) and immunoprecipitated with IgG Dynabeads (For full set of data please refer to Supplementary Tables S4, S5). w/o: Empty vector without CDS fusion. +: positive interaction (luciferase activity ≥ 2.5%). w: weak interaction (luciferase activity = 1.5% ∼ 2.5%). -: no interaction (luciferase activity < 1.5%).