Fig. 3.

Highly glycosylated CD147 promotes secretion of MMPs and decreases tight junction protein amount in BMECs. a BMECs were cultured and stimulated with rt-PA (10 μg/mL) in the presence or absence of AGEs (200 μg/mL for 48 h) and the N-glycosylation inhibitor tunicamycin (TM, 15 μg/mL for 48 h, Calbiochem, San Diego, CA). The cell layers were then subjected to immunoblot assay for CD147 and caveolin-1. A representative blot result is shown. b Densitometric quantification results of caveolin-1. c, d MMPs activities in the conditioned media. e, f Representative immunoblot and densitometric quantification results of occludin and ZO-1. Data expressed as mean ± SEM and analyzed by one-way ANOVA test. One asterisk (*) represents P < 0.05, two (**) P < 0.01, and three (***) P < 0.001. rt-PA for recombinant tissue plasminogen activator; AGEs for glycation end products; MMP for matrix metalloproteinase; ZO-1 for zonula occludens-1