Fig. 5.

Highly glycosylated CD147 promotes MMPs activity in primary astrocytes. a Representative immunoblots of CD147 and caveolin-1 in AGEs and tunicamycin-treated primary astrocytes. b Densitometric quantification results of caveolin-1. c, d MMP-2/9 activity in the conditioned media. One asterisk (*) represents P < 0.05, two (**) P < 0.01, and three (***) P < 0.001. e Representative Western blots of CD147 and caveolin-1 in empty vector- or CD147 N152Q mutant-transfected astrocytes. f, g Densitometric quantification results of CD147 and caveolin-1. h, i MMP-2/9 activity in the conditioned media. Data expressed as mean ± SEM and analyzed by one-way ANOVA. Asterisk (*) denotes comparison with the control group and pound (#) with the empty vector group. rt-PA recombinant tissue plasminogen activator, AGEs glycation end products, TM tunicamycin, EV empty vector, Ctrl control, MMP matrix metalloproteinase, ZO-1 zonula occludens-1