Fig. 2.

Knockdown of circDLST inhibited the proliferation and colony formation of GC cells. a The genomic loci of the DLST gene and circDLST. b qRT-PCR analysis of the expression levels of DLST and circDLST after treatment with RNase R in GC cell lines. c qRT-PCR analysis of the knockdown efficiency of sh-circDLST after the transfection for 48 h in MKN-28 and MKN-45 cells. d MTT analysis of the cell viability after the transfection of sh-circDLST or sh-NC in MKN-28 and MKN-45 cells. e Colony formation analysis of the cell colony number after the transfection of sh-circDLST or sh-NC in MKN-28 and MKN-45 cells. f EdU analysis of the DNA synthesis after the transfection of sh-circDLST or sh-NC in MKN-28 and MKN-45 cells. Bar scale: 125 μm. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01