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. 2019 Apr 5;18:80. doi: 10.1186/s12943-019-1015-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — CircDLST acted as a sponge of miR-502-5p in GC cells. a TCGA analysis of the expression levels of miR-502-5p in paired and unpaired GC tissues. b miRNA expression profiling of miR-502-5p pulled down from the Ago2 or IgG-expressed MKN-45 cells. c, d RIP and qRT-PCR analysis of the amount of circDLST and miR-502-5p pulled down from Ago2 or IgG expressed MKN-45 and MKN-28 cells. e qRT-PCR analysis of the effects of circDLST on miR-502-5p expression levels in MKN-45 and MKN-28 cells. f The luciferase activity of WT and Mut circDLST after co-transfection with PRL-TK-pMIR-Luc-circDLST and miR-502-5p mimic or miR-NC in MKN-45 and MKN-28 cells. g, h FISH analysis of the co-localization of circDLST and miR-502-5p in MKN-45 and MKN-28 cells. Bar scale: 125 μm. Data are the means ± SEM of three experiments. *P < 0.05