Fig. 8.

MiR-502-5p reversed circDLST-induced activation of NRAS/MEK1/ERK1/2 signaling in GC cells. a Schematic representation of potential binding sites of mi-502-5p with WT or Mut NRAS 3’UTR. b The luciferase activity of WT and Mut NRAS 3’UTR after transfection with miR-502-5p inhibitor in MKN-28 cells or miR-502-5p mimic in BGC-823 cells. c, d qRT-PCR analysis of NRAS expression levels after the co-transfection of miR-502-5p inhibitor and sh-circDLST in MKN-28 cells or miR-502-5p mimic and circDLST in BGC-823 cells. e, f Western blot analysis of the activity of NRAS/MEK1/ERK1/2 signaling and PCNA/MMP2 expression after the co-transfection of miR-502-5p inhibitor and sh-circDLST in MKN-28 cells or miR-502-5p mimic and circDLST in BGC-823 cells. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01