Fig. 6.

Functional analysis of selected CypA mutants in Jurkat cells. Jurkat PPIA −/− cells were transduced with lent viral vectors encoding the indicated wild type (WT) and mutant CypA proteins containing the indicated amino acid substitutions. a Analysis of the extent of infection of the indicated cell lines by HIV-1 in the presence and absence of cyclosporin A (CsA). The results shown are from two independent assays. b Analysis of CypA expression by immunoblotting relative to wild type Jurkat cells. Signals were normalized by the corresponding GAPDH signals from the same blot. All samples were separated on the same gel and CypA and GAPDH were stained on the same blot membrane. CypA signals were normalized by the corresponding GAPDH signals