Fig. 4.

IL32 Promoter Activity. a Encode data set, b Promoter region of IL32. TSS determined from 5′RACE are indicated at − 464 bp and − 175 bp from the ATG. Two promoter sites identified by The Eukaryotic Promoter Database are identified as NK4_1 and IL32_1. c Promoter constructs used in luciferase assays. d Promoter constructs were co-transfected with pGL4.73 (Renilla) into melanoma cell lines (M318, M397, and M249). Post-transduction cells were treated for 24 h with 1000 U/mL TNFα and 100 U/mL IFNγ. Luciferase activity was assessed using Dual-Glo according to the manufactures directions. Data was normalized to Renilla luciferase expression and fold changes were calculated against the pGL3 empty control vector. Luciferase assays were performed in triplicate and plotted data is a representative experiment of three independent experiments