FIG 4.

qPCR analysis of the genes related to chitin metabolism. The relative expression levels of the selected genes involved in cell wall integrity and chitin biosynthesis were examined and compared using qPCR. The expression levels of all genes were normalized to the expression level of the endogenous control gene, histone H2A. The expression value of each gene in the control strain was used as the baseline. For qPCR, three replicates were performed for each sample. The average values and standard deviations are represented as bars and error bars, respectively. Con, control strain; ΔdevR, AodevR-disrupted strain; OE-devR, AodevR-overexpressing strain.