FIG 5.

qPCR analysis of the starch degradation-related genes. The strains were cultivated in CD (dextrin) liquid medium at 30°C for 2 days. The relative expression levels of genes involved in starch degradation were examined and compared using qPCR. The expression levels of the genes were normalized to the expression level of the endogenous control gene, the gene encoding histone H2A. The expression value of each gene in the control strain was used as the baseline. For qPCR, three replicates were performed for each sample. The average values and standard deviations are represented as bars and error bars, respectively. Con, control strain; ΔdevR, AodevR disrupted strain; OE-devR, AodevR-overexpressing strain.