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Interactions of DevR with promoter of amyR by yeast one-hybrid assay. Transformed yeast cells were grown on SD-Leu with 50 or 100 ng/ml AbA. The yeast cells that cotransformed with the indicated plasmids [pAbAi-PamyR(−218)/pAbAi-PamyR(−720)/pAbAi-PamyR(−720m) plus pGADT7-devR] were streak cultured onto the plates to examine the growth at 30°C for 3 days. The mutated sequence in the putative binding site (−720m) was used as a negative control. AbA, aureobasidin A.
