TABLE 2.
Primers designed for this study
| Primer | Sequence (5′→3′) | Description |
|---|---|---|
| DH797-F(-980) | GTTCTACTGGCCATCATCGGTCG | Construction of devR gene deletion cassette |
| DH797-R(-1F) | CATCACAGGGTACGTCTGTTGTGATGTGTAAGTGTGGCTTCCATG | |
| pyrG-F | ACAACAGACGTACCCTGTGATG | |
| pyrG-R | AACTGCACCTCAGAAGAAAAGGAT | |
| DH797-F(1670R) | ATCCTTTTCTTCTGAGGTGCAGTTCAATGCAAGGCGTGCAGTACAC | |
| DH797-R(2700) | CAGAATGCTAAATACCCGTGCTC | |
| DH797-F(-900) | GATTTTGTGCCACGTTCCGCTG | |
| DH797-R(2620) | CATTGGACGAGAAGCTCTGGTGAC | |
| OE797-F(IF) | ATTTGAATTCGAATCGATGGAAGCCACACTTACACATCG | Construction of plasmid for devR overexpression |
| OE797-R(IF) | CACGTCGTAGGGGTACCGTCGCTCATCGGTGTACTG | |
| HA-F | TACCCCTACGACGTGCCCGACTAC | |
| Pamy-R | CGATTCGAATTCAAATGCCTTCTGTGG | |
| 797-F(RT) | CCAAACCCCTACAGTATCTTCA | qPCR analysis of AodevR |
| 797-R(RT) | GAGACGGCTGAGAGGACGTAAG | |
| 680-F(RT) | CTCGCTCTTCTTCTCTCTCGAT | qPCR of genes related to chitin metabolism |
| 680-R(RT) | TCGGACATTGGCGAATGCATAG | |
| 353-F(RT) | CCTCCCCGTGTACTACTTTATC | |
| 353-R(RT) | TGGGAGACATGAGAGCCTTTTC | |
| 321-F(RT) | GACGTTGCTGAATGCGTTGCAT | |
| 321-R(RT) | CTCTTCTCCTTGCATGTTCCCA | |
| 1241-F(RT) | CATGTCTTGTCCGCACCATTGA | |
| 1241-R(RT) | ATGAAGAAGGTAGCCTTGGCTC | |
| 944-F | CATGGCTACTGGCAGCAGGATA | qPCR of genes related to starch degradation |
| 944-R | CATATGGTTAGCAACCACATCGAC | |
| 1028-F | TCGGAGTCGTTCACTCGACTAC | |
| 1028-R | CAGGTACTTGAGGAAGACATTGAC | |
| 196-F | GCATCATCGACAAGTTGGAC | |
| 196-R | CGTTCAGAGAGTATATATCCTGC | |
| 746-F | CCTATCATCGAGGAGTTCATTAGC | |
| 746-R | GCTGTCTCGTCGACATTGAACT | |
| 1209-F | GCTCTACCTTAGTCTACGAGA | |
| 1209-R | GTCCATAGATGTTATCGTCAATG | |
| 1498-F | GGTCATCAACAACATGGCATAT | |
| 1498-R | GGTGTAATTGTTCCAGTCAG | |
| AD797-F | GGAGGCCAGTGAATTCATGGAAGCCACACTTACACATCGG | Yeast one-hybrid assay |
| AD797-R | CGAGCTCGATGGATCCCTACCGTCGCTCATCGGTGTACTG | |
| PthiA-F(IF) | AGGAAACAGCTATGAC-TTCGGTAAATACACTATCACA | Construction of plasmid of thiA promoter inducing devR overexpression |
| PthiA-R(FP) | CGATGTGTAAGTGTGGCTTCCAT-GTTTCAAGTTGCAATGACTAT | |
| 797-F(FP) | ATAGTCATTGCAACTTGAAAC-ATGGAAGCCACACTTACACATCG | |
| 797-R(IF) | TACCGAGCTCGAATTC-CTACCGTCGCTCATCGGTGTAC | |
| pUPA-F1 | GAATTCGAGCTCGGTACCCG | |
| pUPA-R1 | GTCATAGCTGTTTCCTGTGTG |