FIG 2.
Effect of 14-Me-C16:Δ2 on QS-regulated functions of B. cenocepacia H111. (A) The chemical structure of 14-Me-C16:Δ2 compared to BDSF. (B) Effects of 14-Me-C16:Δ2 on bclACB gene expression were measured by assessing β-galactosidase activity. (C and D) Quantitative analysis of swarming motility (C) and biofilm formation (D) of B. cenocepacia H111 with the addition of 14-Me-C16:Δ2. Cells were treated with various concentrations of 14-Me-C16:Δ2 and incubated statically at 37°C, and the results are expressed as the relative values compared to those of the untreated controls. The results are based on three independent experiments. Error bars represent the means ± standard deviations (SD). Significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) between the tested compound and its control (n = 3) are shown.