FIG 3.
Effect of 14-Me-C16:Δ2 on the BDSF QS system in B. cenocepacia H111. (A) Effects of 14-Me-C16:Δ2 on rpfFBc gene expression were measured by assessing β-galactosidase activity of the rpfFBc-lacZ transcriptional fusions in the H111 wild-type (WT) strain in the presence or absence of 100 μM 14-Me-C16:Δ2. (B) Quantitative analysis of BDSF production in B. cenocepacia H111 with the addition of 14-Me-C16:Δ2 at different time points. The relative amounts of signal molecules were calculated on the basis of their peak areas. For convenient comparison, the peak BDSF of the WT at 12 h was arbitrarily defined as 100% and used to normalize the signal ratios of the different time points. (C) Quantitative analysis of c-di-GMP levels in B. cenocepacia H111 in the presence or absence of 14-Me-C16:Δ2 (100 μM). The relative amounts of signal molecules were calculated on the basis of their peak areas. For convenient comparison, the peak c-di-GMP of the WT was arbitrarily defined as 100% and used to normalize the signal ratios of the different treatments. The results are based on three independent experiments. Error bars represent the means ± standard deviations (SD). Significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) between the tested compound and its control (n = 3) are shown.