(A and B) Quantitative analysis of swarming motility (A) and biofilm formation (B) of different Burkholderia species in the absence or presence of 14-Me-C16:Δ2 (100 μM). LMG16670, HI2414, J2315, 383, LMG18941, ATCC 17616, LMG14191 and LMG14086 represent B. anthina LMG16670, B. cenocepacia HI2414, B. cenocepacia J2315, B. cepacia 383, B. dolosa LMG18941, B. multivorans ATCC 17616, B. pyrrocinia LMG14191, and B. stabilis LMG14086, respectively. Cells were treated with various concentrations of 14-Me-C16:Δ2 and incubated statically at 37°C, and the results are expressed as the relative values compared to that of the untreated controls. The results are based on three independent experiments. Error bars represent the means ± SD. Significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001) between the tested compound and its control (n = 3) are shown.