TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Phenotypes and/or characteristicsa | Reference or sourceb |
|---|---|---|
| Strains | ||
| Burkholderia spp. | ||
| B. cenocepacia H111 | Wild-type strain, genomovar III of the B. cepacia complex | 42 |
| H111(PcepI-lacZ) | H111 harboring reporter construct PcepI-lacZ | 9 |
| H111(PbclACB-lacZ) | H111 harboring reporter construct PbclACB-lacZ | 11 |
| H111(PrpfFBc-lacZ) | H111 harboring reporter construct PrpfFBc-lacZ | 11 |
| ΔrpfFBc mutant | BDSF-deficient mutant derived from H111 with rpfFBc being deleted | 32 |
| ΔrpfFBc(PcepI-lacZ) mutant | ΔrpfFBc mutant harboring reporter construct PcepI-lacZ | 9 |
| ΔrpfFBc(PbclACB-lacZ) mutant | ΔrpfFBc mutant harboring reporter construct PbclACB-lacZ | 11 |
| B. multivorans ATCC 17616 | Soil, USA | E. Mahenthiralingam laboratory |
| B. cepacia 383 | Soil, Trinidad | E. Mahenthiralingam laboratory |
| B. cenocepacia J2315 | Cystic fibrosis isolate, UK | ATCC |
| B. stabilis LMG 14086 | Respirator, UK | BccMtm |
| B. cenocepacia HI2414 | Isolated from agricultural soil, USA | BccMtm |
| B. dolosa LMG 18941 | Cystic fibrosis isolate, USA | BccMtm |
| B. anthina LMG 16670 | Rhizosphere, UK | BccMtm |
| B. pyrrocinia LMG 14191 | Soil, Japan | BccMtm |
| E. coli | ||
| DH5α | supE44 lacU169(80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 pir | Laboratory collection |
| BL21 | F− ompT hsdS (rB− mB−) dcm+ Tetr gal (DE3) endA | Stratagene |
| Plasmids | ||
| pET-28a | Expression vector, Kanr | Novagen |
| pET-rpfR | pET-28a containing rpfR | 7 |
a
Tetr tetracycline resistance; Kanr, kanamycin resistance.
b
BccMtm, Belgian Coordinated Collections of Microorganisms.