TABLE 2.
PCR primers used in this study
| Primer | Sequence (5′–3′)a |
|---|---|
| For protein expression | |
| rpfR-His-F | CGCGGATCCATGGATGACGAAAACGATAGCGCGG |
| rpfR-His-R | CCCAAGCTTTCAGGCGATCAGCCTGAGCTTTCTC |
| For qPCR assay | |
| BCAL0017-F | AATGAAGACCCTCGCACAGG |
| BCAL0017-R | TCGATATCCGCGTAAAGCCC |
| BCAL0111-F | TCAGCGTAGTGGTTGAACCC |
| BCAL0111-R | TCGGTAATGACGGCGATCAG |
| BCAL0113-F | TTGATCGTGTTCAGCGTCGA |
| BCAL0113-R | AACTTCGCGACGCTCTACAA |
| flhD-F | CTACCAGCGAAATGCTCAGT |
| flhD-R | CATACCCATCGCCTTGTCTT |
| flhC-F | GAAGGAAATCACCCTCGCCA |
| flhC-R | AACAGCGACGAGTGGATGTT |
| flhF-F | ACATTCGCAAATTCACCGGC |
| flhF-R | CGAGTGCAACGATTTCGACG |
| BCAL0248-F | CAATCGCGCAAGTCCTGAAG |
| BCAL0248-R | ATGACCTGCGGAATGTCGTT |
| BCAL0251-F | GTGAAGGGTGGCCGTATTCT |
| BCAL0251-R | TTCTTGAGCGGCACCTTGAA |
| secY-F | CTAACAGCCCGAGTCTTGCA |
| secY-R | AGTTGATCCGGATCGATGCC |
| filI-F | CTCGATCACCGCGTTCTACA |
| filI-R | GATGCGTTTCGTCGATCAGC |
| BCAL0524-F | CAGATGGTGCTCAAGGAAGT |
| BCAL0524-R | GACATGTTCGCGAGGAACT |
| BCAL0566-F | CGGATCCTGGTTCTGCAACT |
| BCAL0566-R | TGACTTCCTCCAACACGACG |
| BCAL0571-F | TGTTGATCGAGATGCCGAGG |
| BCAL0571-R | CTGAAGGATCTCGCGCAGAT |
| BCAL0831-F | TTGCAGGTTGAGTTCGACGA |
| BCAL0831-R | TCCGTATTTGCCCCCGAAAA |
| BCAL0990-F | GCAAAACAAGAAGTCGCCGT |
| BCAL0990-R | ACGACTTTCTTGCCGCGATA |
| BCAL1059-F | CACGCTTCGGCATCTTCAAG |
| BCAL1059-R | CAGGTCGAACGGAATCACCA |
| BCAL1060-F | ATGATGTACATCGCCGCGAA |
| BCAL1060-R | GCATCACTTCCGCGATGAAC |
| leuE-F | TGAGCCTGCTGAATCCGAAG |
| leuE-R | GAAGATCAGCGTGCTCAGGT |
| gtrR-F | GTGAAGGAGGGACTGTTCCG |
| gtrR-R | GACGAACGACTTGAACAGCG |
| zmpB-F | GGAAGGCTTGTCGGAAGG |
| zmpB-R | CCAGTTGTAGACCCAGTGATAC |
| BCAM2374-F | CAATCTGCTCTCCGTCTCCG |
| BCAM2374-R | AATCGGTTCGTAGTGCGTGT |
| BCAM2627-F | CTATCGTCACTGGGTGTCGG |
| BCAM2627-R | CCTTGTGGATCGCATGACCT |
| BCAS0263-F | CGGACGAATTGCTGCACAAG |
| BCAS0263-R | TCGTACTGGTGCAACACGAT |
| BCAS0292-F | GCCGATCGAAGCGGAAAT |
| BCAS0292-R | CAAAGAGCCGGTTGTCGTTG |
| BCAS0638-F | TTGCCGAAGAGAACCTGGTC |
| BCAS0638-R | CCGAGCAGGGTGAAGTGATT |
| BCAL2337-F | TCCACGACAGGAAGTTCACG |
| BCAL2337-R | AAATCTCGATGGGCTTCGCA |
| BCAL2353-F | GTCGTTTCTGGGCAAGGTA |
| BCAL2353-R | CACGTCGTGATCGATGTAGTC |
| BCAL2946-F | GAGCGCACGATCTACATGGA |
| BCAL2946-R | AGGAAGTGATAGCCGATGCG |
| BCAL2978-F | GAACGTGTCGATGTGCATGG |
| BCAL2978-R | TCGGCTACGACATGCACTAC |
| BCAL3041-F | CGATGGTCATCAACGGCAAG |
| BCAL3041-R | GTTCTTCTCGCCCTTCTGCT |
| BCAL3405-F | TACAACATCGGCAAGCAGGT |
| BCAL3405-R | GGCAACTGCTCGTAGGTGAT |
| bclB-F | CTTTACCCACGACGACCTTTAC |
| bclB-R | TCGTATTGCGGCAGTTTCTC |
| BCAM0193-F | TATCGGGAAGGCAGCTACT |
| BCAM0193-R | CCGTAGTAATGGATTTCGAGCA |
| BCAM0196-F | GGAGCCTGATCCATACGTCG |
| BCAM0196-R | CATCGCCATGTCGAGAAAGC |
| BCAM0854-F | GGGACGATGGCGATTTCTT |
| BCAM0854-R | GGTTCCATCACCGCATAGTC |
| BCAM0859-F | TCGCTACAACGATCTCGACG |
| BCAM0859-R | CTTGCCTTCGAGCGAGATCT |
| BCAM0862-F | GCAACAAGTTCTACGCGACC |
| BCAM0862-R | GTCGAAGCTGCCGTATTTGC |
| BCAM0948-F | GCATGATGATGAGCAAGCCG |
| BCAM0948-R | CCCAGTAGTCCGGATAACGC |
| BCAM1004-F | TGAACTACCGTGAATCGTATGG |
| BCAM1004-R | CAACGGTGTCCGTGATCTT |
| BCAM1005-F | GCGTCAGGTAGTAGATCGGC |
| BCAM1005-R | CGGTCGAGGAGCAGTTCTAC |
| BCAM1010-F | GTGGGAAGAGGACATCATCAAG |
| BCAM1010-R | CGAAGATCGTCGGCATGAATA |
| BCAM1745-F | GCGATGTCGATCAGGTTCTT |
| BCAM1745-R | TGGCCGTCATGTTCAGGTAC |
| BCAM1870-F | AGTTCGATCGCGACGATACC |
| BCAM1870-R | AGCGACTTCAGCAGATACGG |
| BCAM1871-F | CTCGAACGACAGGTTGACGA |
| BCAM1871-R | GTATTTGCTGCGCATCTCCG |
| BCAM2044-F | CGGTCACGTAAAGCTCGGTA |
| BCAM2044-R | ACGTTTACGATCGGCTTCCA |
| BCAM2060-F | GTTGAGCAGGAACAGGTCGA |
| BCAM2060-R | GTGCTGTACGTGAACCAGGA |
| BCAM2140-F | GCGACATCGCATTCATTCATC |
| BCAM2140-R | ATCGTGTCGGGCGAAATC |
| bapA-F | CTGTTGTTGGTGCGATCATTT |
| bapA-R | CAACGTCGTGCCGTCATA |
| BCAM2215-F | AATCGGTCGTTGACGGGAAA |
| BCAM2215-R | CTTCAGCGTGAACGTGTAGC |
| BCAM2225-F | TCGCTCAAACGTTTGCAGTG |
| BCAM2225-R | GAACCATGCGCGGATCAAC |
| aidA-F | GACGTTGTCCTGGTTGGTCA |
| aidA-R | CGCGTTACCGATGTACTCGT |
a
Restriction enzyme sites are underlined.