Figure 6.

Bcor insufficiency causes the activation of myeloid-related genes. (A) Principal component (PC) analyses based on total gene expression in LSK HSPCs, GMPs, and CD71⁺Ter119⁺erythroblasts isolated from WT, ΔE9-10, ΔTet2, and DKO MDS mice. (B) GSEA performed using RNA sequence data. Summary of GSEA data and GSEA plots of representative data are shown. Normalized enrichment scores (NES), nominal P values (NOM), and false discovery rates (FDR) are indicated. The gene sets used are indicated in supplemental Table 2. Quantitative reverse transcription–PCR analysis of Cebpa and Cebpe in LSK cells (C), Hoxa7 and Hoxa9 in GMPs (D), and Cdkn1a and Bax in CD71⁺Ter119⁺ erythroblasts (E). Hprt1 was used to normalize the amount of input RNA. Data are shown as the mean ± SEM (n = 3). Statistical significance is shown relative to WT. *P < .05; **P < .01; ***P < .001 by the Student t test.