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. Author manuscript; available in PMC: 2019 Oct 10.
Published in final edited form as: Cell Host Microbe. 2018 Oct 10;24(4):542–557.e9. doi: 10.1016/j.chom.2018.09.006

Fig 3: The TALOS element in CrPV-1A shows relaxed specificity for interaction with Ago-2.

Fig 3:

(A) List of amino acid insertions and deletions introduced into the CrPV-1A flexible loop. (B) Effects of insertions and deletions on TALOS element-mediated RNAi suppression and Ago-2 interaction were measured using the RNAi reporter assay and Western blot analysis, respectively. (C) Effects of mutation of F114 and P106 for their ability to suppress RNAi and interact with Ago-2 were probed by RNAi-reporter assay and Western blot analysis, respectively. RNAi suppression data in (B) and (C) represent mean (±SD) of at least three independent experiments (n = 3) for each condition. Western blots analysis in (B) and (C) shows one of three representative experiments (n = 3).

See also Fig S2