| REAGENT or RESOURCE |
SOURCE |
IDENTIFIER |
| Antibodies |
| Mouse monoclonal ANTI-FLAG M2 |
Sigma Life Science |
Cat# F3165; RRID:AB_259529 |
| Rabbit polyclonal Anti-Argonaute-2 (Drosophila) |
Abcam |
Cat# ab5072; RRID:AB_2096294 |
| Mouse monoclonal Anti-beta Actin |
Abcam |
Cat# ab8224; RRID:AB_449644 |
| Ubiquitin Remnant Motif (K-ε-GG), Academic license |
Cell Signaling Technology |
Cat #5562 |
| Mouse monoclonal Anti-alpha Tubulin |
Santa Cruz |
Cat #sc-8035; RRID:AB_628408 |
| Rabbit polyclonal CrPV anti-3CD |
Garrey et al., 2010 |
N/A |
| Rabbit polyclonal CrPV anti-VP2 |
Garrey et al., 2010 |
N/A |
| Goat Anti-Rabbit IgG, Texas Red |
Thermo Fisher Scientific |
Cat #T6391; RRID:AB_10374713 |
| Bacterial and Virus Strains |
| CrPV-3 |
Kerr et al., 2015 |
N/A |
|
Escherichia coli BL21(DE3) |
John D Gross Lab |
N/A |
|
Escherichia coli B834(DE3) |
Novagen |
Cat# 69041 |
| SURE 2 Supercompetent Cells |
Agilent Technologies |
Cat# 200152 |
| Chemicals, Peptides, and Recombinant Proteins |
| L-Selenomethionine |
Anatrace |
Cat# S2000 |
| Sequencing Grade Modified Trypsin, Frozen |
Promega |
Cat# V5113 |
| Phusion High Fidelity DNA Polymerase enzyme |
New England Biolabs |
Cat# M0530L |
| Dynabeads M-270 Epoxy |
Thermo Fisher |
Cat# 14301 |
| EZview Red ANTI-FLAG M2 Affinity Gel |
Sigma-Aldrich |
Cat# F2426 |
| EZview Red Protein A Affinity Gel |
Sigma-Aldrich |
Cat# P6486 |
| MG-132 proteasome inhibitor |
Sigma-Aldrich |
Cat# C2211 |
| cOmplete Mini EDTA-free Protease Inhibitor tablets |
Roche |
Cat# 11836170001 |
| Ni-NTA Agarose |
QIAGEN |
Cat# 30210 |
| Dynabeads His-Tag Isolation and Pulldown |
Thermo Fisher Scientific |
Cat# 10103D |
| 3X FLAG Peptides |
Sigma-Aldrich |
Cat# F4799 |
| Critical Commercial Assays |
| Dual-Luciferase Reporter Assay System |
Promega |
Cat# E1910 |
| Vaccinia Capping System |
New England Biolabs |
Cat# M2080S |
| SensiFAST SYBR No-ROX Kit |
BIOLINE |
Cat# BIO-98005 |
| Pierce Silver Stain Kit |
Thermo Fisher Scientific |
Cat# 24612 |
