FIG 3.

Inhibition of cell fusion of cells transiently expressing MeV F and MeV receptors. HEK 293T cells were cotransfected with either MeV IC323-WT F (A, C) or MeV IC323-F L454W (B, D), α-subunit of β-galactosidase, and wt IC323 MeV H. Transfected cells were subsequently overlaid 3 h posttransfection with HEK 293T cells expressing the indicated receptors (nectin-4, SLAM/CD150, or no receptor; only for F L454W, since wt F does not mediate fusion in the absence of receptor) and the ω-subunit of β-galactosidase in the presence of decreasing concentrations of HRC4 (A, B) or 3G (C, D). Cells were then incubated overnight to permit fusion. Resulting luminescence from β-galactosidase activity was quantified using Tecan Infinite M1000 Pro. Results depict means and standard errors of the means (SEMs) from two (3G) or three (HRC4) biological replicates of three technical replicates each. Dotted lines indicate 50% inhibition.