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TG and brain stem infection of C57BL/6 mice with HSV-1 McKrae. C57BL/6 female mice were infected with HSV-1 McKrae as described in Materials and Methods. Tissues from at least three mice were harvested at the indicated times during acute infection. TG (black) and brain stem (blue) samples were homogenized (whole) to determine infectious virus titers. The red circle indicates samples obtained from mice (n = 3) displaying signs of encephalitis (e.g., hunched posture) on day 10 p.i.
