FIG 3.

Viral proteins were not detected in the brain stem post-hyperthermic stress. C57BL/6 female mice were infected with McKrae. (A) At 8 dpi, mice displaying symptoms of encephalitis were sacrificed, and the brain stems were processed for the detection of viral proteins (brown) and counterstained with cresyl violet. The box shows the area at a higher magnification. (B and C) Latently infected mice were induced to reactivate by hyperthermic stress. At 24 h phs, the brain stems and TG from six mice were harvested and processed for the detection of viral proteins. Viral proteins were not detected in the brain stem (B), but positive neurons (brown) were identified in the TG by whole-ganglion immunohistochemistry (WGIHC) (C).