TABLE 1.

Recovery of infectious virus^a

Parameter	BS, 20 PFU	BS, 20 PFU	BS, 20 PFU	TG, 20 PFU	BS, 75 PFU	TG, 75 PFU	BS, 150 PFU	BS, 150 PFU	BS, 150 PFU	TG, 150 PFU
Input PFU	21	21	21	21	77	77	154	154	154	154
Recovered PFU	8	2	6	8	9	29	70	57	43	97
	4	11	7	5	12	21	83	70	57	78
	6	4	4	10	21	18	83	90	50	80
	6	5	5	4	17	27	33	93	90	31
	4	15	6	6	33		50	40	80	52
	1	4	6		24		47	43	73
	8	7			22		67	103
	4	4			26		50	80
	3	4			23		67	30
	8	5			26		137	77
	11	7			21		87	63
	7	3			27		47	57
Mean tissue wt (g) ± SD	0.0114 ± 0.0020	0.0107 ± 0.0022	0.0139 ± 0.0048	0.0155 ± 0.0047	0.0119 ± 0.0046	0.0137 ± 0.0035	0.0119 ± 0.0013	0.0113 ± 0.0014	0.0103 ± 0.0007	0.0167 ± 0.0078
Mean % recovery ± SD	27.78 ± 13.13	28.17 ± 17.64	26.98 ± 4.92	31.43 ± 11.47	28.24 ± 8.56	30.85 ± 6.65	44.42 ± 17.87	43.45 ± 14.74	42.53 ± 11.92	43.89 ± 16.89

^aUninfected brain stems were divided into six parts and individually weighed. TG pairs were also harvested and weighed. Each brain stem (BS) or TG sample was spiked with approximately 20, 75, or 150 PFU of 17VP16pLZ and homogenized as described in Materials and Methods. The input PFU were also directly plated alongside tissue samples onto RSC. Each BS column represents an independent experiment. Recovery in paired TG was pooled from 2 to 3 experiments. Plates were stained with crystal violet 2 to 3 days later, and the numbers of PFU recovered were counted. The number of PFU recovered in each sample was divided by the average PFU detected by directly plating the input virus, and the results are presented as the mean percent recovery ± the standard deviation for each experiment.